![]() ![]() 8ĭata were processed using Excel software and DPS v7.05 software, and significance of difference was analyzed using Duncan's new multiple range method. ![]() 7 Root samples were collected at 35 d after seeding, to determine infection rate, and mycorrhizal infection rate was calculated according to frequency standard method. At 60 d post inoculation, the fifth functional leaves at the same position of cucumber plants were selected from each treatment the photosynthetic indexes, such as photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO 2 concentration (Ci) and transpiration rate (Tr), were measured with LI-6400 photosynthetic apparatus chlorophyll content was determined using acetone method root activity was determined using TTC method. Ten seedlings were sampled at 36 d after seeding the aerial part and underground part of seedlings were taken, rinsed with tap water to remove surface debris, then rinsed with deionized water after removed moisture, the fresh weight was measured, and the average fresh weight per plant was obtained afterwards, the samples were deactivated at 105℃ for 15 min, and dried at 75℃ to constant weight the samples were weighed, to get average dry weight per plant. Plant height (height from cotyledon to growing point) and stem diameter (the thickness of cotyledon section parallel to cotyledon expanding direction) of seedlings were measured at 32, 39, 46 and 53 d after seeding, respectively. Equal volume of sterilized inoculums was applied in CK, to ensure that trace elements were consistent. ![]() The 50-hole trays were used for nursery, which were wiped with 70% alcohol and dried 10% (160g) mycorrhizal inoculums were applied in each treatment inoculums were mixed with substrates and loaded into trays, 1600g each tray. On February 4, germinated cucumber seeds were sown in trays and inoculated with inoculums. Nursery substrates were prepared with peat and vermiculite at the proportion of 2V:1V the substrates were processed in air dry oven at 160℃ for 2h, and the process was repeated one more time the substrates were preserved for later use when cooled naturally. Cucumber seeds were soaked in warm water at 55℃ for 10 min, then soaked in normal temperature water for 8h, and finally accelerated germination in an incubator at 30℃. Three treatments were designed in the test, including VT inoculation, BF inoculation and CK (non-inoculation), and each treatment was repeated three times. Cucumber seedlings trial#The trial was performed in the solar greenhouse of Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences in the spring of 2015. The inoculums contained 800-1000 mycorrhizal spores per gram. claroideum BEG 210), were provided by professor Vosatka Miroslav at Institute of Botany, Czech Academy of Sciences. rhizophagus, produced by Czech Symbiom Coopearation), and plant universal inoculum BF (mycorrhiza inoculum for plants, in abbreviation, the mixed strain of G. The cucumber variety used in the test was Zhongnong 16, breed by Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences special commodity compound inoculums for vegetables, VT (mycorrhiza inoculum for vegetable, VT in abbreviation, the mixed strain of Glomus claroideom, G. Seedling growth and photosynthetic physiological indexes of cucumber were measured, in order to screen commodity AMF mixture inoculums suitable for cucumber nursery in China. Two AMF mixture inoculums introduced from Czech Republic were applied in cucumber nursery. 6 There is no commodity mixture inoculum suitable for domestic cucumber seedling in production. The inoculum composed of different strains can be used for seedling inoculation, which is featured by simple operation, low cost and low volume. 1 Although the symbiotic effect between single AMF strain and cucumber has been reported 2−5 different AMF strains can influence the metabolic process of plant through different modes and approaches after symbiosis. ![]() Arbuscular mycorrhizal fungi (AMF) is a kind of soil microbes that is symbiotic with plant roots, which has a variety of functions as follows: promoting plant growth, improving Rhizosphere environment, improving plant absorption of beneficial nutrients P, K, Ca, Cu and Fe by extending hyphae, increasing plant resistance to adverse environment, defending harms of other harmful microbes on plant, and improving yield and quality of crops. ![]()
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